Metabolite characterisation and profiling of <i>Hermetia illucens</i> L. larvae at various growth stages using <i>Sesamum indicum</i> residues as nutrient source

The larvae of black soldier fly (Hermetia illucens L.) (BSFL) is an excellent decomposer for industrial and agricultural applications capable converting low-value waste into nutritional biomass. Sesame residues food possess high protein lipid content. To investigate the bioconversion process BSFL, were raised on a low-bioburden diet consisting sesame residue, liquid chromatography-mass spectrometry (LC-MS) analysis was performed BSFL faeces at various instars. obtained metabolomes subsequently evaluated through principal component orthogonal projection to latent structure-discriminant explore differential components. Diacylglycerol main utilised by during metabolism. phenylalanine, arginine, oleic acid 10-hydroxy-8E-octadecenoic identified as major components in instars metabolites. We thus concluded that have different compositions instar stages. Another compound with molecular weight 278.224 prevalent both might play vital roles development. present study revealed part metabolic changes important lifecycle. Through this pioneering metabolomics profiles, discovery highly-valuable biomolecules application can be anticipated.